Biochemical and Biophysical Research Communications Intermediates in Flavoprotein Catalyzed Hydroxylations

INTERMEDIATES IN FLAVOPROTEIN CATALYZED HYDROXYLATIONS Barrie Entsch, Vincent Massey and David P. Ballou Department of Biological Chemistry, The University of Michigan Ann Arbor, Michigan 48104 Received November 21,1973 Summary The reaction of molecular oxygen with the complex of reduced p-hydroxybenzoate hydroxylase and 2,4-dihydroxybenzoate has been followed by rapid reaction techniques. During the reaction, which produces stoichiometric amounts of oxidized enzyme and the hydroxylated product,2,3,4-trihydroxybenzoate, three spectroscopically distinguishable intermediates have been detected and characterized.